The filtration device invented by Professor Yvon Cayre is part of a process of conceptual simplicity of his own. This simplicity was made possible by incorporating elements of high technology resulting in a true "technological breakthrough" when compared to other tools developed for the isolation of rare cells from blood. The filtration device comprises, as shown below:
(1) a tank in which is loaded the peripheral blood sample obtained from the patient in a standard EDTA tube;
(2) a very innovative super-hydrophilic polyethylene glycol diacrylate filter, biocompatible polymer for obtaining a very "flat" surface with conical pores which do not allow accumulation of normal blood cells as occurs in cylindrical pores once the filtration is completed; a “vortex” located in the lower end of the reservoir allows the blood to reach the filter horizontally to create a " cross flow " avoiding the accumulation of normal blood cells on the surface;
(3) a polymer wick crossed vertically by multiple micro - super- hydrophilic channels for capillarization of blood passing through the filter.
Once filtration is completed, tumor cells (or fetal cells) with a size greater than that of normal blood cells are retained on the surface of the filter. The filter is then ejected from the upper part of the filtration device and serves as a support for all techniques of interest for identification and characterization of isolated cells as well as detection of their molecular characteristics.